Category MI L23 Don't Sweat the Small Stuff

Abstract The purpose of this experiment was to determine which odor eliminating

spray killed the most bacteria that were extracted from a previously worn

shoe to determine whether the sprays successfully killed the bacteria or

simply masked the odor. Oust, Febreeze, and Dr.Scholl's odor eliminating

sprays were tested on the shoe bacteria. It was originally hypothesized

that Dr. Scholl's would kill the most bacteria, but it was later hypothesized

that Oust would kill the most bacteria after several tests. To test this

experiment, filter paper was sterilized in an autoclave for thirty minutes

and placed in a shoe during exercise. The filter paper was then cut into 1

cm by 1 cm pieces, sprayed with each odor- eliminating sprays, and

diluted in water using sterilized equipment. The diluted water was applied

to three triptych soy agar dishes per spray with a graduated pipette and

placed in an incubator for twenty-four hours. After the twenty-four

hours, the samples were placed in a refrigerator until ready to be

observed. The number of bacterial colonies that formed on each of the

agar dishes was counted, compared, and averaged to determine which

spray had the least amount of bacterial growth. Oust proved best

effective in killing the sweat bacteria, and Febreeze proved to be the least

effective in killing bacteria. This experiment accurately proves that though

air fresheners and odor eliminators may have a pleasant aroma and say

that bacteria is killed through the use of the spray, most do not actually kill

the bacteria that is the cause of the foul smell.

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